Comparative Analysis of HPLC Fingerprint Combined with DAD Detection between Bupleuri Radix and Bupleuri Herba
نویسندگان
چکیده
منابع مشابه
Comparative Analysis of HPLC Fingerprint Combined with DAD Detection between Bupleuri Radix and Bupleuri Herba
Abstract Bupleuri Herba is widely distributed and traditionally used for the similar clinical application of Bupleuri Radix in Jiangsu and Anhui province in China. To elucidate the difference of material basis between Bupliuri Radix and Bupleuri Herba, a HPLC fingerprint method combined with DAD detection was developed for effective components analysis of these two herb medicines. Both flavonoi...
متن کاملSpecies classification and quality assessment of Chaihu (Radix Bupleuri) based on high-performance liquid chromatographic fingerprint and combined chemometrics methods.
A high-performance liquid chromatographic (HPLC) method was established to analyze 36 Chaihu (Radix Bupleuri) samples collected from three species (Bupleurum chinense DC., B. scorzonerifolium Willd. and B. smithii Wolff.). Addition of trifluoroacetic acid into the mobile phase resulted in fingerprint chromatograms with stable baselines. There were thirty-two characteristic peaks in the standard...
متن کاملRadix bupleuri Extract Inhibits Hyperplasia of Mammary Gland in Rats
Purpose: To investigate the effect of Radix bupleuri extract (RBE) on hyperplasia of mammary gland (HMG) in rats. Methods: Forty virgin female Wistar rats were randomly divided into control, HMG model, positive control (Rupisanjie capsule, RPSJC), and low-, middle-, and high-dose RBE groups. Estrogen and progestogen were intramuscularly injected into the rats to induce HMG. RPSJC and RBE were a...
متن کاملClerosterol from vinegar-baked radix bupleuri modifies drug transport
Vinegar-baked Radix Bupleuri (VBRB) is reportedly used to treat liver cancer when combined with traditional chemotherapy and data show that this combination may modify drug transport. We isolated clerosterol from VBRB and studied its effect on drug transporters in normal or transporter-overexpressing cells. Transporter activity was assayed using cellular substrate concentration and transporter ...
متن کاملAnalysis of Radix Bupleuri (Chaihu) using Agilent 1200 Series LC Systems with Evaporative Light Scattering Detector
This Application Note describes the development of conventional HPLC and Rapid Resolution LC (RRLC) methods for the analysis of Buplerui (Chaihu). A comparison of fingerprint chromatograms of different Buplerui samples showed different peak profiles of different TCMs collected from different places. The method for the Agilent 1200 Series Evaporative Light Scattering Detector (ELSD) was optimize...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Food and Nutrition Research
سال: 2015
ISSN: 2333-1119
DOI: 10.12691/jfnr-3-4-6